Achievements

Till now, we have collected a total of 2,499 samples from pig farms, 1,527 samples from slaughtering houses, and 1,317 samples from terminal markets. Among these samples, we have detected E.coli, Salmonella, and S. suis in these samples with a variety of prevalence. We have also tested the antimicrobial susceptibility of 763 E. coli and 312 Salmonella isolates so far. We found that 93.1% of the E. coli isolates from pig farms were resistant to more than three classes of the antibiotics tested. In comparison, 66.4% and 67.5% of the isolates from slaughtering houses and markets were multidrug-resistant, respectively. We also discovered that over 50% of the Salmonella isolates were resistant to ampicillin, amoxicillin, spectinomycin, tetracycline, doxycycline, florifenicol, erytromycine, tilmicosin, sulfisoxazole, and sulfamethoxazole. We genotyped 401 E. coli isolates recovered from pig farms (n=191), slaughtering houses (n=140), and markets (n=70). We found that sequence types (STs) ST10, ST101 were recovered from samples of pig farms, slaughtering houses, and markets.

We have also isolated six lytic phages that could be used to treat E. coli. We isolated 16 lytic phages that could be used to mitigate Salmonella.

We also developed a rapid method for simultaneous detection of E. coli O157:H7 and Salmonella Typhimurium in 2 h.

Published seven research papers and presented 28 papers at different meetings/seminars.